CD3 epsilon antibody

Catalog No. ABIN931331

The Mouse Monoclonal anti-CD3 epsilon antibody has been validated for FACS, WB and ELISA. It is suitable to detect CD3 epsilon in samples from Human.

Quick Overview for CD3 epsilon antibody (ABIN931331)

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Clone: 4E2

Product Details anti-CD3 epsilon Antibody

Immunogen: CD3 E antibody was raised in Mouse using a purified recombinant fragment of CD3 expressed in E. coli as the immunogen.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10,000, FC: 1:200-1:400, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Store at -20 °C for long term storage.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Synonyms: Monoclonal CD3E antibody, Anti-CD3E antibody, CD3 epsilon antibody.

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway